Dr. Ann Kleinschmidt
B214 Steffee
Office Phone 332-2366
332-5330
Office Hours
MW 10 AM - Noon
TTh 9:30 - 1:45
or By Appointment through WebCalendar

Dr. Doros Petasis
Carr 123
Office Hours
MWF 10-11
TTh 3:00-4:30
or by appointment
(dpetasis@allegheny.edu)

Dr. Lenny Vuocolo

Goals for Biochemistry 580
Develop proficiency with primary literature and scientific review articles
Understand the interdisciplinary nature of biochemical research
Understand the functioning of a particular class of enzymes, heme-containing enzymes
Exposure to faculty doing Biochemical research at Allegheny College
Understanding the context of biochemical research, how to carry out research in a socially responsible manner
Develop proficiency with using the scientific search tools and writing review papers
Develop proficiency with use of molecular biological, biochemical, bio-inorganic, and biophysical techniques for carrying out a defined project.

Date Topic Assignment Class Time
F Jan 16 Introduction to the Course 2:30
M Jan 19 Introduction to peroxidase proteins/Computer work Read Papers about Peroxidase/ Ascorbate Peroxidase 2:30
F Jan 23 Introduction of non-protein peroxidases 2:30
M Jan 26 Ways of analyzing peroxidase/Bug Buster Read Papers about Analysis of PeroxidaseRead Bug Buster Protocl 1:30
F Jan 30 Ni-NTA column for peroxidase Read Ni-NTA column protocol 1:30
M Feb 2 Concentration/Absorbance/Heme addition Read protocol for electrophoresis/concentratio/Addition of Heme 1:30
F Feb 6 DEAE column, measuring absorbance, concentrating Read protocol for DEAE column/Measuring absorbance 1:30
M Feb 9 Effects of mutations on Peroxidase activity Read Papers on effect of mutations on peroxidase/Discussion of design of mutations 2:30
F Feb 13 Theory of EPR Read Brudvig's paper from "Methods in Enzymology" and "The Essence of EPR" handout 2:30
M Feb 16 Instrumentation Read "EPR Spectroscopy in Enzymology" handout and Protocol for EPR spectrometer operation 2:30

F Feb 20 Papers for EPR Read Cammack & Cooper and Yonetami papers 2:30
M Feb 23 Analysis of Purified Peroxidase using EPR Read Poulos et al paper 2:30
F Feb 27 Measuring Enzymatic Activity/Gel electrophoresis/Spectrophotometric Read Protocol for analysis of peroxidase protein activity 1:30
M Mar 1 Discussion of Writing Lab Paper, Library Work 1:30
F Mar 5 Faculty visit Visit Faculty re comps 2:30
M Mar 8 Faculty visit Visit Faculty re comps 2:30
F Mar 12 Brainstorming/Discussion of Careers Visit Faculty re comps 2:30
M Mar 15 Spring Break Relax No meeting
F Mar 19 Spring Break Relax No Meeting
M Mar 22 Library Work Determine topic of review paper 2:30
F Mar 26 Synthesis of meso-tetraphenylporphyrin (TPP) 1:30
M Mar 29 Analysis of TPP and synthesis of Fe(Cl)TPP 1:30
F Apr 2 UV/visible and EPR spectrometric analysis of non-protein peroxidases 2:30
M Apr 5 Determination of complexes' peroxidase activity 1:30
F Apr 9 Demonstration of peroxidase kinetics 1:30
M Apr 12 NMR analysis 1:30
F Apr 16 To be announced
M Apr 19 No Class, Attend Senior Symposium
F Apr23 Student Presentations 2:30
M Apr 26 Student Presentations 2:30

ASSESSMENT FOR CLASS
2 Experimental Research Papers 20% each 40%
Review Paper 20% 20 %
Class Participation 20 % 20%
Lab Participation 10% 10 %
Paper Presentation 10% 10%
Total 100%